A sensitive, reproducible and rapid reverse phase high performance liquid chromatographic methods were developed and validated for the determination of fluconazole (FLZ) in presence of its degradation products in pure form and pharmaceutical formulations Grace Smart C18 RP Column (250 × 4.6 mm, 5 μm) was used with a mobile phase containing a mixture of acetonitrile:water in the ratio of (60:40). The flow rate was 0.8 ml/min and effluents were monitored at 261 nm and eluted at 3.83 min. Calibration curve was plotted with a range from 10-50 µg/ml. The method was validated for the parameters like accuracy, precision, robustness and system suitability parameters. The precision, ruggedness and robustness values were also within the prescribed limits (< 1% for system precision and < 2% for other parameters). The LOD and LOQ were found to be 0.5216 μg/ml and 1.580 μg/ml respectively.
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